Collagen-Induced Arthritis Model (CIA)
Rheumatoid arthritis (RA) is an autoimmune disease associated with chronic inflammation, primarily in the joints. RA is characterized by synovial inflammation, swelling, and autoantibody production, which result in the destruction of cartilage and bone in the small joints of hands, wrists, and feet. Vium offers a well-established rat collagen-induced arthritis (CIA) model against which to validate the efficacy of potential therapeutic compounds (1-2). In the rat CIA model, both B and T cells mediate joint inflammation and destruction with signature increases in the cytokines IL-1, IL-6, IL-17, and TNF-alpha which are validated targets for development for new therapeutics.
Vium Arthritis IndexTM
The Arthritis Index provides an objective measure of function-related disease severity comparable to the traditional arthritis score. The Arthritis Index is automatically scored using a computer algorithm removing the variability that may arise from manual measurements.
Available Conventional Measures
- Arthritis score
- Ankle joint size (caliper)
- Ankle joint histopathology
We have validated our CIA model in both rats and mice following standard protocols. Disease onset occurs between 11 to 15 days with average day of onset occurring between study day 12 & 13. Our induction rate is > 95%.
Subjects are randomized into groups using motion prior to induction and disease severity at time of onset.
Subjects are enrolled into treatment groups following the onset of a significant Vium Arthritis Index score or an arthritis score of > 1. A standard study may dose for 14 – 28 days.
Treatment efficacy is assessed using Vium metrics and optional conventional measures to compare disease severity between vehicle and treated animals. Study progress, subject videos and graphs are made available to you securely via your account on Vium's Research Suite.
Positive control therapeutics
Several positive control therapeutics can be used including:
- Methotrexate (daily, PO)
- Enbrel (q3d, SC)
- Dexamethasone (daily, PO or IP)
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- Bevaart L, Vervoordeldonk MJ, Tak PP. (2010) Evaluation of therapeutic targets in animal models of arthritis: how does it relate to rheumatoid arthritis? Arthritis Rheum. 62: 2192-205.
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- Courtenay JS, Dallman MJ, Dayan AD, Martin A, Mosedale B. (1980) Immunisation against heterologous type II collagen induces arthritis in mice. Nature 283: 666-8.